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. 2021 Jul 8;28(1):1455–1465. doi: 10.1080/10717544.2021.1949075

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Cell uptake and cytotoxicity of NPs. (A) After RAW264.7 cells were incubated with coumarin-6 loaded NPs for 2 h and then stained with 4% paraformaldehyde, fluorescent images were observed by confocal microscopy. Scale bar: 50 μm. (B) The fluorescent images were observed using FCM. The data are presented as the means ± SD (n = 3). *indicates p < .05. (C) Cell viability of RAW264.7 cells incubated for 72 h with different concentrations of free-DMSA, DMSA-NLC, and AEM-DMSA-NPs. The data are presented as the means ± SD (n = 3).