Neural induction medium (d0 - d7)
mTesr Custom		StemCell Technologies;
PenStrep (100x)	1x	Life Technologies; 15070063
SB431542	10 μM	Stemgent; 04-0010
LDN193189	250nM	Stemgent; 04-0074
Retinoic acid	100nM	Sigma-Aldrich; R2625
Basal medium
DMEM/F12, GlutaMAX		ThermoFisher; 10565018
PenStrep (100x)	1x	Life Technologies; 15070063
2-Mercaptoethanol (1000x)	1x	Life Technologies; 21985023
MEM non-essential amino acids (NEAA) solution (100x)	1x	Life Technologies; 11140-050
N2 medium (d8 - 11)
Basal medium
N2 supplement (100x)	1x	Life Technologies; 15070063
Retinoic acid	100nM	Sigma-Aldrich; R2625
Smoothened agonist (SAG)	1μM	EMD Millipore; 566660
N2B27 medium (d12 - 19)
Basal medium
N2 supplement (100x)	1x	Life Technologies; 15070063
B27 Supplement without VitA (50x)	1x	Life Technologies; 12587-010
Insulin solution, human	25μg/mL	Sigma-Aldrich; 19278
Retinoic acid	100nM	Sigma-Aldrich; R2625
Smoothened agonist (SAG)	1μM	EMD Millipore; 566660
PDGF medium (d20 – sort)
Basal medium
N2 supplement (100x)	1x	Life Technologies; 15070063
B27 Supplement without VitA (50x)	1x	Life Technologies; 12587-010
Insulin solution, human	25μ/mL	Sigma-Aldrich; 19278
PDGFaa	10ng/mL	R&D Systems; 221-AA-050
IGF-1	10ng/mL	R&D Systems; 291-G1-200
HGF	5ng/mL	R&D Systems; 294-HG-025
NT3	10ng/mL	EMD Millipore; GF031
T3	60ng/mL	Sigma-Aldrich; T2877
Biotin	100ng/mL	Sigma-Aldrich; 4639
cAMP	1μM	Sigma-Aldrich; D0260
Glial medium (post-sort)
Basal medium
N2 supplement (100x)	1x	Life Technologies; 15070063
B27 Supplement without VitA (50x)	1x	Life Technologies; 12587-010
Insulin solution, human	25μg/mL	Sigma-Aldrich; 19278
T3	60ng/mL	Sigma-Aldrich T2877
Biotin	100ng/mL	Sigma-Aldrich 4639
cAMP	1μM	Sigma-Aldrich D0260
HEPES	10mM	Sigma-Aldrich H4034
Ascorbic acid	20μg/mL	Sigma-Aldrich A4403