TABLE 1.
Mutagenesis and insertion analysis for conserved residues in TMD of E1a
| Single mutation(s) | Double mutations | Insertion |
|---|---|---|
| K174: A/E/Δ | K174A and R177A (pYM-39) | (i) Between T166 and A167 LLALLA insertion |
| R177: A/E/K/Δ | R177E and Q182A | (ii) Between G178 and Q179 LLALLA insertion |
| G178: L | G178L and G183L | |
| Q179: N/E/A/Δ | Q182A and G183A (pYM-42) | |
| Q182: N/A/Δ | ||
| G183: L |
a
The original amino acid is given in the first column; the positions in the E1 protein, followed by the different substitutions that were tested, are also shown in one-letter code. Δ = deletion of amino acid.