FIG 1.

Anti-NHR antibodies have a lower level of somatic hypermutation than HIV-1 bNAbs. (A) Percentages of mutated amino acids across both heavy- and light-chain V genes from germline are reported for eight bNAbs (black) representative of the four main bNAb epitopes (MPER, V1/V2 region, V3 glycan, and CD4bs) and four monoclonal antibodies against the NHR (blue). Sequences of the antibodies involved in this analysis were previously reported (17, 54–56, 60–67). This analysis included the full sequence of the framework regions 1, 2, and 3 and CDR1 and −2. (B) Comparison of percent amino acid mutation between bNAbs (using the eight bNAbs presented in panel A) and anti-NHR antibodies; bNAbs have a mean somatic hypermutation of 25% compared to the 9.5% for anti-NHR antibodies. Each dot represents a unique antibody’s percent amino acid mutation, with error bars denoting standard errors of the means. The percentages of amino acid mutations from germline are reported on the y axis, as the published sequences cited above mostly reported only amino acid sequences.