FIG 2.

Recombination of known beneficial CDR sequences to engineer novel D5 variants. (A) Mutated CDR sequences of four highlighted D5 variants reported by Montgomery et al. (67) aligned with the wild-type (WT) sequence. These mutated CDR sequences were used to engineer the 11 recombined variants. (B) Left, paratope map of the binding sites for D5 Fab with its antigen, 5-helix. NHR, yellow; CHR, light blue. Areas of contact with the D5 CDR loops are represented as VH CDR1 (red), VH CDR2 (green), VH CDR3 (magenta), and VL CDR3 (blue). Right, X-ray crystal structure of D5 in complex with 5-helix (PDB 2CMR) (59). (C) Schematic of D5 variants engineered and tested. The identity of each of the four CDR loops is represented by 0 (WT) or 1 (mutant).