FIG 3.

D5_AR Fab has a greater binding affinity to CCIZN17, an NHR-mimetic, than D5 Fab. The binding affinity (reported numerically as the K_D) of D5 (red) and D5_AR (blue) Fab for CCIZN17, a variation of a previously described NHR mimetic (58, 79), is presented as association and dissociation curves using biolayer interferometry. The K_Ds are reported as the means from fitted values across multiple concentrations from at least two independent experiments and are reported with the standard errors of the means.