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. 2021 Jul 12;95(15):e02350-20. doi: 10.1128/JVI.02350-20

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Copyright © 2021 Rubio et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — The neutralization potencies of D5_AR IgG against tier-2 HIV-1 viruses are substantially higher in TZM-bl cells expressing FcγRI. (A) Neutralization curves demonstrating the enhanced potency of D5_AR IgG against lentivirus pseudotyped with HIV-1 HXB2 (tier-1B) and 25710 (tier-2) in TZM-bl cells expressing FcγRI versus TZM-bl cells without FcγRI. Data points and error bars are means and standard errors of the means (n = 2), respectively. The neutralization curves presented in TZM-bl/FcγRI cells plateaued at around 90% neutralization compared to the 100% neutralization reported in TZM-bl cells; this is possibly explained by varying levels of FcγRI expression on the TZM-bl/FcγRI cells. (B) ID₅₀ values and fold enhancement of neutralization potency of D5_AR IgG against lentivirus pseudotyped with HIV-1 HXB2 (tier-1B) and 25710 (tier-2) in TZM-bl cells versus that in TZM-bl/FcγRI cells. D5_AR is potentiated approximately 1,000-fold in TZM-bl/FcγRI cells. (C) ID₅₀ values for D5_AR against a panel of tier-2 HIV-1 viruses in TZM-bl/FcγRI cells. Many of the viruses had ID₅₀ values below the limit of detection (0.023 μg/ml). (D) ID₅₀ values for D5_AR against another panel of tier-2 and SHIV challenge viruses in TZM-bl/FcγRI cells but with a lower limit of detection.