TABLE 2.
Primers and probes used for ddPCR
| Primer/probe namea | Sequence |
|---|---|
| HIV-gag; Fwd primer | TCTCGACGCAGGACTCG |
| HIV-gag; Rev primer | TACTGACGCTCTCGCACC |
| HIV-gag; probe | FAM-CTCTCTCCTTCTAGCCTC-MGBNFQ |
| RPP30; Fwd primer | GATTTGGACCTGCGAGCG |
| RPP30; Rev primer | GCGGCTGTCTCCACAAGT |
| RPP30; probe | VIC-CTGAACTGAAGGCTCT-MGBNFQ |
| HIV multiplex primer/probe | sequence |
| HIV-gag; Fwd primer | TCTCGACGCAGGACTCG |
| HIV-gag; Rev primer | TACTGACGCTCTCGCACC |
| HIV-gag; probe | FAM-CTCTCTCCTTCTAGCCTC-IABkFQ |
| HIV-env (RRE); Fwd primer | AGTGGTGCAGAGAGAAAAAAGAGC |
| HIV-env (RRE); Rev primer | GTCTGGCCTGTACCGTCAGC |
| HIV-env (RRE); probe | HEX- CCTTGGGTTCTTGGGA-IABkFQ |
| HIV-env (RRE); hypermutant probe | IABkFQ-CCTTAGGTTCTTAGGAGC-IABkFQ |
| House-keeping multiplex Primer/Probe | |
| RPP30-shearing; Fwd primer | CCATTTGCTGCTCCTTGGG |
| RPP30-shearing; Rev primer | CATGCAAAGGAGGAAGCCG |
| RPP30-shearing; probe | FAM-GGAAAGGAGCAAGGTTC-IABkFQ |
| RPP30; Fwd primer | GATTTGGACCTGCGAGCG |
| RPP30; Rev primer | GCGGCTGTCTCCACAAGT |
| RPP30; probe | HEX-CTGAACTGAAGGCTCT-IABkFQ |
a
Fwd, forward; Rev, reverse.