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Determined parameters of mobile phase gradient conditions in a HPLC separation of FMOC-derivatized amino acids.
| Program time [min] | Flow rate [ml min–1] | %A | %B |
| 1.3 | 62.0 | 38.0 | |
| 5 | 1.3 | 62.0 | 38.0 |
| 12 | 1.3 | 43.0 | 57.0 |
| 14 | 1.3 | 24.0 | 76.0 |
| 15 | 1.3 | 43.0 | 57.0 |
| 18 | 1.3 | 620 | 38.0 |
Mobile phase consists of elution buffer 50 mM Na-acetate pH = 4.2 (A) and organic solvent acetonitrile (B).
