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. Author manuscript; available in PMC: 2021 Aug 12.
Published in final edited form as: Sci Immunol. 2021 Feb 12;6(56):eabb9435. doi: 10.1126/sciimmunol.abb9435

Figure 1. MO influx and Res cDC TZ relocalization during Type 1 inflammation.

Figure 1.

A-B) B6 mice were injected in the ears with the indicated adjuvants and dLNs were analyzed 24h later by histocytometry. A) Representative images depicting Imaris 3D surfaces for indicated cell types, overlayed with B220 staining. White circles denote the TZ boundary. [See fig. S1A for gating]. B) Frequency of Res cDCs and MOs within the TZ, as well as MO density. Data analyzed using Brown-Forsythe and Welch ANOVA tests with Dunnett’s multiple comparison. C-D) B6 mice were immunized with CpG in both ears and dLNs were analyzed at indicated time points. C) Histocytometry for Res cDC localization and MO density. D) MOs in dLNs enumerated by flow cytometry. Data representative of 5 independent experiments. E-F) B6 mice were infected in the footpad with 100 pfu of West Nile virus-TX and dLNs were analyzed by histocytometry. E) Representative images from mock infected and 36h post infection. F) Localization of Res cDCs and MO density at the indicated time points. Scale bars denote 200 μm. Data represent at least 2 independent experiments.