Figure 2. Surveyor Assay confirmation of multiplex gene targeting using lipoMSN.

(A) Schematic illustrating the roles of Pcsk9, Apoc3 and Angptl3 on lipid metabolism. Surveyor assays showing in vitro editing of (B) pcsk9, (C) apoc3, and (D) and angptl3 was durable despite lowered doses of target gRNA. (E) Gene editing efficiency quantification using Image J. Results are presented as average ± SEM (n = 4). Positive control of Lipofectamine CRISPRMax delivering targeted RNP noted by ‘+’, while negative control of Cas9 protein only noted with ‘-’. Control and singular target (1) RNP was delivered at 2 ug/mL, while target-specific RNP for dual-targeting (2) was at 1 ug/mL each (2 ug/mL total) and triple-targeting (3) was at 0.67 ug/mL (2 ug/mL total).