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. 2021 Jul 13;5(8):e620. doi: 10.1097/HS9.0000000000000620

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Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the European Hematology Association.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal.

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Figure 4. — Fab-format LRPAP1 BAR-bodies—binding assays. Flow cytometric binding assay to identify the optimal conformation for Fab-format LRPAP1 BAR-bodies. MAVER1 cells were stained with 2 clones of Fab-format BAR bodies versions A, B, and C (A, C, and E). Granta-519 mantle cell lymphoma cells were used as controls (B, D, and F). Fab-format BAR-bodies of version A showed the most intensive staining reaction of MAVER1 cells and no detectable binding to Granta-519 cells (A and B). The conformation of the Fab-format BAR-body, version A, was therefore chosen for the further development of IgG1-format LRPAP1 BAR-bodies. 5 × 10E6 lymphoma cells (MAVER1 or Granta-519) were incubated with Fab-format BAR-bodies (5 µg/mL). APC labeled anti-His antibody was used for detection. BAR=B-cell receptor antigens for reverse targeting.