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. 2021 Jul 13;5(8):e620. doi: 10.1097/HS9.0000000000000620

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Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the European Hematology Association.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal.

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Figure 5. — Binding of IgG1-format LRPAP1 BAR-bodies to NK cells. NK cells were isolated from PBMCs by magnetic depletion of all non-NK cells using the CD56+/CD16+ human NK-Cell Isolation Kit (Miltenyi Biotech GmbH). αCD16 antibodies were FITC-coupled and αCD56 antibodies were PE-coupled. The viability of the NK cells after isolation averaged 95% and the CD16+ fraction was between 90 and 98% (dot plot on the left). IgG1-format LRPAP1 BAR-bodies (histogram on the right) but not αCD3/LRPAP1 bispecific BAR-bodies (histogram in the middle) showed binding to isolated NK cells. Mouse Anti-Flag and APC-coupled anti-mouse antibodies were used as secondary system. BAR=B-cell receptor antigens for reverse targeting; PBMC=peripheral blood mononuclear cell.