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. 2021 Jul 5;14:2955–2962. doi: 10.2147/JIR.S310116

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© 2021 Zhang et al.

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Increased pyroptosis was observed in hyperosmotic stress (HS)-triggered human corneal epithelial cells (iHCECs) in vitro, while inhibition of pyroptosis by disulfiram could protect cells from HS-induced cell cytotoxicity. (A) Cell viability of iHCECs treated with various concentrations of disulfiram (2, 4, 8 μM). (B) Disulfiram treatment (8 μM) suppressed lactate dehydrogenase (LDH) release in HS-stimulated cells. (C) Disulfiram treatment (8 μM) reduced the proportion of pyroptotic cells, shown as Caspase-1/PI double positive by flow cytometry. (D) Relative protein level of N-GSDMD in blank, HS, and disulfiram treated groups. *P<0.05, **P<0.01.