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. 2021 Jul 5;14:2955–2962. doi: 10.2147/JIR.S310116

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© 2021 Zhang et al.

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Calcitriol suppressed the aggregation of ASC speck formation, and inhibited the NLRP3–ASC–caspase-1–GSDMD pyroptosis pathway. (A) Immunofluorescence images of ASC speck formation (white arrows) in HS-stimulated iHCECs treated with/without calcitriol (ASC, red; nuclei, blue; ×200 magnification). Analysis of three independent experiments assessing ASC speck formation to calculate the percentage of cells containing ASC speck. (B) The levels of pyroptosis-related proteins including NLRP3, cleaved Caspase-1, N-GSDMD, and cleaved IL-1β were detected by western blotting, and quantification of the bands is shown in the right panel. *P<0.05, **P<0.01, ***P<0.001.