Table 4. In vivo responses to amastigotes in the spleen of infected mice using LDU assay (mean ± σ, n = 5).

Group	Spleen wt. (mg)	Amastigotes per 500 nuclei	LDU (× 104)a	% of amastigote inhibitionb	% of amastigote replication suppressionc
Before treatment	130 ± 10	410 ± 60
Infected control	180 ± 10	2580 ± 610	45 ± 13
BPQ treated	100 ± 10	200 ± 20	2 ± 1	54 ± 13	93 ± 1
BPQ+NP treated	80 ± 10	27 ± 3	0.3 ± 0.1*	94 ± 4*	99 ± 1*

^a LDU (Leishman Donovan Unit) = number of amastigotes per 500 nuclei × spleen weight in mg.

^b Calculated using Eq (3)

^c Calculated using Eq (4)

* p < 0.01, significant compared to BPQ.