Fig 3. Lipid screening of Tm-PPase by nanoDSF.

(a) F₃₃₀ signal of the no lipids reference sample (black line) and a representative set of stabilising lipids (coloured lines) during the melting scan. Grey lines show the fluorescence traces of the other tested lipids. (b) First derivative of the F₃₃₀ signal highlighting the fluorescence change indicative of protein unfolding. The T_m can be determined at the curve peak, here shown for the no lipids reference sample as dotted line. (c) ΔT_m of detergent-solubilised to relipidated sample shown for all tested lipids, which are sorted and colour coded according to their lipid class. Panel a-b show the traces of a representative nanoDSF scan. Panel c shows the ΔT_m and corresponding SEM based on three technical repeats. The asterixis represent the p-value (* = 0.0332, ** = 0.021, *** = 0.002, **** = 0.0001) obtained by a one-way ANOVA followed by a Dunnet test to correct for multiple comparison in GraphPadPrism 7.