Table 1. Lipid screen design containing 31 different lipids or lipid mixtures.
| Well | Lipid | Abbreviation | Amount | Comment | References |
|---|---|---|---|---|---|
| A1 | 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine | DOPE | 0.3 mg | Phosphatidylethanolamine (PE) | [43] |
| A2 | |||||
| A3 | |||||
| A4 | 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphoethanolamine | POPE | 0.3 mg | Phosphatidylethanolamine (PE) | [12] |
| A5 | |||||
| A6 | |||||
| A7 | 1,2-Dipalmitoyl-sn-Glycero-3-Phosphoethanolamine | DPPE | 0.3 mg | Phosphatidylethanolamine (PE) | [43] |
| A8 | |||||
| A9 | |||||
| A10 | 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphocholine | POPC | 0.3 mg | Phosphatidylcholine (PC) | [44] |
| A11 | |||||
| A12 | |||||
| B1 | 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine | DOPC | 0.3 mg | Phosphatidylcholine (PC) | [45] |
| B2 | |||||
| B3 | |||||
| B4 | 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine | DMPC | 0.3 mg | Phosphatidylcholine (PC) | [46] |
| B5 | |||||
| B6 | |||||
| B7 | 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphocholine | DPPC | 0.3 mg | Phosphatidylcholine (PC) | [47] |
| B8 | |||||
| B9 | |||||
| B10 | 1-Myristoyl-2-Hydroxy-sn-Glycero-3-Phosphocholine | LMPC | 0.3 mg | Phosphatidylcholine (PC) | [48] |
| B11 | |||||
| B12 | |||||
| C1 | 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphoglycerol (sodium Salt) | POPG-Na | 0.3 mg | Phosphoglyceride (PG) | [49] |
| C2 | |||||
| C3 | |||||
| C4 | 1,2-Dipalmitoyl-sn-Glycero-3-Phospho-1’-rac-Glycerol (sodium salt) | DPPG-Na | 0.3 mg | Phosphoglyceride (PG) | [49] |
| C5 | |||||
| C6 | |||||
| C7 | 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phospho-L-Serine (sodium salt) | POPS | 0.3 mg | Phosphatidylserine (PS) | [50] |
| C8 | |||||
| C9 | |||||
| C10 | 1,2-Dioleoyl-sn-Glycero-3-Phospho-L-Serine (sodium salt) | DOPS-Na | 0.3 mg | Phosphatidylserine (PS) | - |
| C11 | |||||
| C12 | |||||
| D1 | 1,2-Didecanoyl-sn-Glycero-3-Phosphate (sodium salt) | 10:PA-Na | 0.3 mg | Phosphatic acid (PA) | [51] |
| D2 | |||||
| D3 | |||||
| D4 | 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphate (sodium salt) | 16:0–18:1 PA (POPA) | 0.3 mg | Phosphatic acid (PA) | - |
| D5 | |||||
| D6 | |||||
| D7 | E. coli Polar Lipid Extract | EPL | 0.3 mg | Mixture of PE (67%), PG (23.2%) and CA (9.8%) | [52] |
| D8 | |||||
| D9 | |||||
| D10 | Cholesteryl Hemisuccinate Tris Salt | CHS | 0.3 mg | Sterol | [53] |
| D11 | |||||
| D12 | |||||
| E1 | Cholesterol | CHL | 0.3 mg | Sterol | [54] |
| E2 | |||||
| E3 | |||||
| E4 | 1’,3’-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (sodium salt) | 14:0 CL | 0.3 mg | Cardiolipin (CL) | [13] |
| E5 | |||||
| E6 | |||||
| E7 | 1’,3’-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (sodium salt) | 18:1 CL | 0.3 mg | Cardiolipin (CL) | [13] |
| E8 | |||||
| E9 | |||||
| E10 | Porcine Polar Brain Lipid Extract | PBL | 0.3 mg | Mixture of PC (12.6%), PE (33.1%), PI (4.1%), PS (18.5%), PA (0.8%) and unknown (30.9%) | [55] |
| E11 | |||||
| E12 | |||||
| F1 | Egg Sphingomyelin (chicken) | SM | 0.3 mg | Mixture of sphingomyelins (SM) with 16:0 SM (86%), 18:0 SM (6%), 22:0 SM (3%), 24:1 SM (3%), unknown (2%) | [56] |
| F2 | |||||
| F3 | |||||
| F4 | Monomyristolein | 7.7MAG | 0.3 mg | Monoacylglycerol | [57] |
| F5 | |||||
| F6 | |||||
| F7 | Monoolein | 9.9MAG | 0.3 mg | Monoacylglycerol | [58] |
| F8 | |||||
| F9 | |||||
| F10 | Lipid mix I | POPC:POPG: POPE (3:1:1)* | 0.3 mg | Used for structure solution of several ion channels | [59–61] |
| F11 | |||||
| F12 | |||||
| G1 | Lipid mix II | POPC:POPS (4:1)* | 0.3 mg | Simple mimic of the membrane inner lipid leaflet | - |
| G2 | |||||
| G3 | |||||
| G4 | Lipid mix III | POPG:POPE (3:1)* | 0.3 mg | Simple mimic of Gram-positive bacterial membranes | - |
| G5 | |||||
| G6 | |||||
| G7 | Lipid mix IV | POPG:POPE (1:3)* | 0.3 mg | Simple mimic of Gram-negative bacterial membranes | - |
| G8 | |||||
| G9 | |||||
| G10 | Lipid mix V | DMPC:CHL (2:1)* | 0.3 mg | Simple mimic of eukaryotic plasma membranes | - |
| G11 | |||||
| G12 | |||||
| H1 | Lipid mix VI | POPC:CHL (5:1)* | 0.3 mg | Simple mimic of nerve cell membranes | - |
| H2 | |||||
| H3 | |||||
| H4 | Lipid mix VII | CHL:POPC:SM (1.8:1:1)* | 0.3 mg | Simple mimic of erythrocyte membranes | - |
| H5 | |||||
| H6 | |||||
| H7 | Lipid mix VIII | POPC:POPE (1:1)* | 0.3 mg | Simple mimic of mitochondrial membranes | - |
| H8 | |||||
| H9 | |||||
| H10 | Blank | Blank | 0 mg | Control | - |
| H11 | |||||
| H12 |
* molar ratios.