Figure 2.
Dose-dependent effect of LTA in PBMECs. Effect of lipoteichoic acid on SOD1 and Gpx1 antioxidant enzyme and COX2 expressions measured in PBMECs to determine the suitable LTA dose by qRT-PCR (A–C). For optimum LTA dose confirmation, the expression of COX2 protein was measured in PBMECs stimulated with LTA (100 μg/mL) (D). Glyceraldehyde-3 phosphate dehydrogenase (GAPDH) is used as a control. Graphs show the densitometric analysis (protein/GAPDH) from three independent experiments. Values represent means ± SEM. LTA, lipoteichoic acid. Different uppercase letters (A–D) on the top of bars indicate significant differences (n = 3, p < 0.05) among the groups.