Table 1.

Sequence of primers used in this study.

Genes	Sequence (5′-3′)	References
Gpx1	F: CCCCTGCAACCAGTTTGG, R: GAGCATAAAGTTGGGCTCGAA
SOD1	F: GGCTGTACCAGTGCAGGTCC, R: GCTGTCACATTGCCCAGGT
COX2	F: CGTTTTCTCGTGAAGCCCTATG, R: CTCCATGGCATCTATGTCTCCAT
GAPDH	F: GGGTCATCATCTCTG CACCT, R: GGTCATAAGTCCCTCCACGA	(29)

The reverse transcription–generated cDNA encoding Gpx1 (glutathione peroxidase 1), SOD1 (superoxide dismutase-1), COX2 (cyclooxygenase 2), GAPDH (glyceraldehyde-3 phosphate dehydrogenase) was amplified by RT-PCR using the above selective primers. F, forward primer (5′-3′); R, reverse primer (3′-5′).