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. 2021 Jun 28;8:661380. doi: 10.3389/fvets.2021.661380

Table 1.

Sequence of primers used in this study.

Genes Sequence (5-3) References
Gpx1 F: CCCCTGCAACCAGTTTGG, R: GAGCATAAAGTTGGGCTCGAA
SOD1 F: GGCTGTACCAGTGCAGGTCC, R: GCTGTCACATTGCCCAGGT
COX2 F: CGTTTTCTCGTGAAGCCCTATG, R: CTCCATGGCATCTATGTCTCCAT
GAPDH F: GGGTCATCATCTCTG CACCT, R: GGTCATAAGTCCCTCCACGA (29)

The reverse transcription–generated cDNA encoding Gpx1 (glutathione peroxidase 1), SOD1 (superoxide dismutase-1), COX2 (cyclooxygenase 2), GAPDH (glyceraldehyde-3 phosphate dehydrogenase) was amplified by RT-PCR using the above selective primers. F, forward primer (5′-3′); R, reverse primer (3′-5′).