| Antibodies |
|
|
| APC-eFluor 780 anti-CD8 (clone 53-6.7) |
eBioscience Thermo Fisher |
Cat#: 47-0081-82; RRID:AB_1272185 |
| PE-Cy7 anti-CD4 (clone GK1.5) |
eBioscience Thermo Fisher |
Cat#: 25-0041-82; RRID: AB_ 469575 |
| FITC anti-TCRβ (clone H57-597) |
eBioscience Thermo Fisher |
Cat#: 11-5961-82; RRID: AB_ 465322 |
| PE-Cy7 anti-TCRβ (clone H57-597) |
eBioscience Thermo Fisher |
Cat#: 25-5961-82; RRID:AB_ 2573506 |
| APC anti-CD44 (clone IM7) |
eBioscience Thermo Fisher |
Cat#:17-0441-81; RRID:AB_469389 |
| eFluor 660 anti-Eomes (clone Dan11mag) |
eBioscience Thermo Fisher |
Cat#: 50-4875-82; RRID:AB_ 2574226 |
| APC anti-RORγt (clone AFKJS-9) |
eBioscience Thermo Fisher |
Cat#: 17-6988-82; RRID:AB_ 1633425 |
| APC anti-IL-4 (clone 11B11) |
eBioscience Thermo Fisher |
Cat#: 17-7041-82; RRID:AB_469493 |
| APC anti-IL-17 (clone eBio17B7) |
eBioscience Thermo Fisher |
Cat#: 17-7177-81; RRID:AB_ 763580 |
| eFluor 660 anti-T-bet (clone eBio4B10) |
eBioscience Thermo Fisher |
Cat#: 50-5825-82; RRID:AB_ 10596655 |
| eFluor 660 anti-Foxp3 (clone FJK-16 s) |
eBioscience Thermo Fisher |
Cat#: 50-5773-82; RRID:AB_ 11220082 |
| PE anti-CD25 (clone PC61.5) |
eBioscience Thermo Fisher |
Cat#: 12-0251-82; RRID:AB_ 465606 |
| PE-Cy7 anti-CD25 (clone PC61.5) |
eBioscience Thermo Fisher |
Cat#: 25-0251-82; RRID:AB_ 469607 |
| PE anti-CD69 (clone H1.2F3) |
eBioscience Thermo Fisher |
Cat#: 12-0691-82; RRID:AB_ 465731 |
| APC-eFluor 780 anti-CD45 (clone 30-F11) |
eBioscience Thermo Fisher |
Cat#: 47-0451-82; RRID:AB_ 1548790 |
| PE anti-Bcl-xL (clone 54H6) |
Cell Signaling Technology |
Cat#: 13835S; RRID:AB_ 2228008 |
| PE isotype control (clone DA1E) |
Cell Signaling Technology |
Cat#: 5742S; RRID:AB_1550038 |
| Alexa Fluor 647 anti-PLZF (clone R17-809) |
BD Biosciences |
Cat#: 563490; RRID:AB_2738238 |
| FITC anti-CD122 (clone TM-β1) |
BD Biosciences |
Cat#: 553361; RRID:AB_ 394808 |
| PE anti-CD124 (clone mIL4R-M1) |
BD Biosciences |
Cat#: 552509; RRID:AB_ 394407 |
| Alexa Fluor 647 anti-RORγt (clone Q31-378) |
BD Biosciences |
Cat#: 562682; RRID:AB_2687546 |
| PE anti-Bcl-2 (clone 3F11) |
BD Biosciences |
Cat#: 556537; RRID:AB_396457 |
| PE Bcl-2 isotype control (clone A19-3) |
BD Biosciences |
Cat#: 556537; RRID:AB_470073 |
| PE anti-Vδ 6.3/2 (clone 8F4H7B7) |
BD Biosciences |
Cat#: 555321; RRID:AB_395729 |
| PE anti-TCRγδ (clone GL3) |
BD Biosciences |
Cat#: 553178; RRID:AB_394689 |
| Alexa 647 anti-TCRβ (clone H57-597) |
BioLegend |
Cat#: 109218; RRID:AB_ 493346 |
| APC anti-IFNγ (clone XMG1.2) |
BioLegend |
Cat#: 505810; RRID:AB_ 315404 |
| PE anti-PLZF (clone 9E12) |
BioLegend |
Cat#: 145804; RRID:AB_ 2561973 |
| PE-Cy7 anti-CD24 (clone M1/69) |
BioLegend |
Cat#: 101822; RRID:AB_ 756048 |
| APC anti-Vγ1.1 (clone 2.11) |
BioLegend |
Cat#: 141108; RRID:AB_ 10901177 |
| CD1d tetramers (PBS-57 loaded) |
NIH tetramer facility |
Emory University, Atlanta, Georgia |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| LIVE/DEAD Fixable Aqua Dead Cell Stain Kit |
eBioscience Thermo Fisher |
Cat#: L34957
|
| PMA |
Sigma Aldrich |
Cat#: P8139 |
| Ionomycin |
Sigma Aldrich |
Cat#: I0634 |
| Brefeldin A |
eBioscience Thermo Fisher |
Cat#: 00-4506-51 |
| QuantiTect SYBR® Green PCR Kits |
QIAGEN |
Cat#: 204145 |
| RNeasy Plus Micro kit |
QIAGEN |
Cat#: 74034 |
| QuantiTect reverse transcription kit |
QIAGEN |
Cat#: 205313 |
| Percoll |
GE Life Sciences |
Cat#: 17089101 |
| Critical Commercial Assays |
|
|
| eBioscience Fixation/Perm diluents |
eBioscience Thermo Fisher |
Cat#:00-5223-56 |
| eBioscience Fixation/Perm concentrate |
eBioscience Thermo Fisher |
Cat#:00-5213-43 |
| Permeabilization Buffer 10x |
eBioscience Thermo Fisher |
Cat#: 00-8333-56 |
| Experimental Models: Organisms/Strains |
|
|
| Mouse: C57BL/6 (C57BL/6NCrl) |
Charles River Laboratories |
Stock#: 24107773, 24107757 |
| Mouse: BALB/c (BALB/cAnNCrl) |
Charles River Laboratories |
Stock#: 24107700, 24107684 |
| Mouse: PLZFGFPcre (B6(SJL)-Zbtb16
tm1.1(EGFP/cre)Aben/J) |
(Constantinides et al., 2014) The Jackson Laboratory |
Stock#: 024529 |
| Mouse: PLZF-deficient mice (PLZF−/−) |
Barna et al., 2000 |
Provided by Dr. P.P. Pandolfi (Harvard Medical School, Boston, MA) |
| Oligonucleotides |
|
|
| RT-PCR Zbtb16-Forward: 5′-TTGGGACGGACCCACACCCA-3′ |
This paper |
N/A |
| RT-PCR Zbtb16-Reverse: 5′-GGCGTGGCCGGTGAATAGGG-3′ |
This paper |
N/A |
| RT-PCR Rpl13-Forward: 5′-CGAGGCATGCTGCCCCACAA-3′ |
This paper |
N/A |
| RT-PCR Rpl13-Reverse: 5′-AGCAGGGACCACCATCCGCT-3′ |
This paper |
N/A |
| Genotype Zbtb16 WT Forward: 5′-GCAGATCTGGGACCACCATC-3′ |
This paper |
N/A |
| Genotype Zbtb16 WT Reverse: 5′-GCTGCATACAGCAGGTCATC-3′ |
This paper |
N/A |
| Genotype Zbtb16 KO Forward: 5′- GGTGCCAATGTCACTTATAGAACC-3′ |
This paper |
N/A |
| Genotype Zbtb16 KO Reverse: 5′- GCTTCCTCGTGCTTTACGGTATC-3′ |
This paper |
N/A |
| Genotype PLZFGFP/CRE WT Forward: 5′-CTCCTCCATGCAGAAACACA-3′ |
This paper |
N/A |
| Genotype PLZFGFP/CRE Reverse: 5′-TAGTGAAACAGGGGCAATGG-3′ |
This paper |
N/A |
| Genotype PLZFGFP/CRE Rep Forward: 5′-CCCCAGGAAATAATCCAAGG-3′ |
This paper |
N/A |
| Software and Algorithms |
|
|
| GraphPad Prism 7 |
GraphPad software |
N/A |
| FlowJo software version 10.2 |
FlowJo |
N/A |
| Active Control 4.2.0.7 |
Division of Computer Research and Technology, NIH |
N/A |
| Other |
|
|
| Canvas X |
Canvas GFX |
N/A |
| BD FACS LSRII |
BD Biosciences |
N/A |
| BD FACS LSRFortessa |
BD Biosciences |
N/A |
