FIGURE 2.

The kinetics and heterogeneity of Perforin⁺CD45⁺ cells in the ischemic brain. (A) The percentages of Perforin⁺CD11b⁺CD45^low microglia, Perforin⁺CD11b⁺CD45^high macrophages, and Perforin⁺CD11b^–CD45^high lymphocytes in the ischemic brain were measured by flow cytometry. CD3⁺NK1.1^– cells was derived from perforin⁺CD11b^–CD45^high lymphocytes and divided into CD4⁺ and CD8⁺ T cells. (B) The numbers of perforin⁺CD45⁺ lymphocytes, macrophages, and microglia after ischemic stroke at these time points. (C) The numbers of NK, NKT, CD3^–NK1.1^–, and CD3⁺NK1.1⁺ cells among perforin⁺CD11b^–CD45^high lymphocytes after ischemic stroke at these time points. (D) The numbers of CD4⁺CD8^–, CD4^–CD8⁺, CD4^–CD8^– and CD3⁺ TCRγδ⁺ cells among perforin⁺CD11b^–CD45^high lymphocytes after ischemic stroke at these time points. (E) Flow cytometry showed the ability of Aqua^–CD11b⁺CD45^low microglia to secrete perforin. (F) The proportion of Prf1-GFP among Aqua^–CD11b⁺CD45^low microglia after dMCAO. The data are shown as the mean ± SEM; n = (6–10) mice per group.