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. 2021 Jul 5;13:5337–5350. doi: 10.2147/CMAR.S311242

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© 2021 Xie et al.

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Circ_0003221 silence inhibited cervical cancer cell progression in vitro. SiHa and CaSki cells were transfected with si-NC or si-circ_0003221. (A) The expression of circ_0003221 was examined by qRT-PCR. (B and C) CCK-8 assay was used for detecting cell viability. (D) Colony formation assay was performed to examine the number of colony cells. (E and F) Flow cytometry analysis was used to determine cell cycle distribution. (G) Cell proliferation ability was determined by Edu assay (×200). (H and I) Western blot assay was conducted to analyze the protein expression of PCNA and Cyclin D1. (J and K) Transwell assay was conducted to estimate cell migration and invasion (×100). (L and M) The protein levels of E-cadherin, Snail and Vimentin were determined by Western blot assay. *P<0.05.