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. 2021 Jul 5;13:5337–5350. doi: 10.2147/CMAR.S311242

Figure 4.

Figure 4

Knockdown of circ_0003221 inhibited the progression of cervical cancer cells by upregulating miR-758-3p. (A) The expression of miR-758-3p was measured by qRT-PCR in SiHa and CaSki cells transfected with anti-miR-NC or anti-miR-758-3p. (B–K) SiHa and CaSki cells were transfected with si-NC, si-circ_0003221, si-circ_0003221 + anti-miR-NC, or si-circ_0003221 + anti-miR-758-3p. (B and C) CCK-8 assay was applied to detect cell viability. (D) Colony formation assay was used for measuring cell colony formation ability. (E and F) Cell cycle distribution was analyzed via flow cytometry analysis. (G) Cell proliferation ability was examined using Edu assay (×200). (H) Western blot assay was performed to detect the protein expression of PCNA and Cyclin D1. (I and J) Cell migration and invasion abilities were assessed by transwell assay (×100). (K) E-cadherin, Snail and Vimentin protein levels were determined by Western blot assay. *P<0.05.