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. 2021 Jul 5;13:5337–5350. doi: 10.2147/CMAR.S311242

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© 2021 Xie et al.

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CPEB4 was a downstream target of miR-758-3p. (A) Bioinformatics databases were used to predict the potential target genes of miR-758-3p. (B) The expression of potential target genes was detected by qRT-PCR in normal tissues and cervical cancer tissues. (C) The complementary binding sites of miR-758-3p and CPEB4 3ʹUTR were shown. (D–G) Dual-luciferase reporter assay and RIP assay were performed to verify the interaction between miR-758-3p and CPEB. (H and G) The mRNA and protein expression of CPEB4 were respectively determined by qRT-PCR and Western blot in in SiHa and CaSki cells transfected with anti-miR-NC, anti-miR-758-3p, miR-NC, or miR-758-3p. (J and K) CPEB4 mRNA and protein expression in normal tissues and cervical cancer tissues were detected by qRT-PCR and Western blot, respectively. (L) The correlation between CPEB4 mRNA expression and miR-758-3p expression was analyzed in cervical cancer tissues. (M and N) The mRNA and protein levels of CPEB4 in ECT1/E6E7, SiHa and CaSki cells were measured by qRT-PCR and Western blot, respectively. *P<0.05.