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. 2021 Jul 5;13:5337–5350. doi: 10.2147/CMAR.S311242

Figure 6.

Figure 6

Overexpression of miR-758-3p repressed cervical cancer cell progression by downregulating CPEB4. (A and B) The mRNA and protein levels of CPEB4 in SiHa and CaSki cells transfected with pcDNA or pcDNA-CPEB4 were measured by qRT-PCR and Western blot, respectively. (CN) SiHa and CaSki cells were transfected with miR-NC, miR-758-3p, miR-758-3p + pcDNA, miR-758-3p + pcDNA-CPEB4. (C and D) Cell viability was measured using CCK-8 assay. (E and F) Cell cycle distribution was measured using flow cytometry analysis. (G) Colony formation assay was performed to measure colony formation ability. (H) Edu assay was used to assess cell proliferation ability (×200). (I and J) Western blot assay was conducted to examine the protein levels of PCNA and Cyclin D1. (K and L) Cell migration and invasion were tested using transwell assay (×100). (M and N) The protein levels of E-cadherin, Snail and Vimentin were determined by Western blot assay. *P<0.05.