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. 2021 Jul 6;15:2907–2919. doi: 10.2147/DDDT.S307670

Figure 9.

Figure 9

Tetrandrine repressed the PI3K/Akt signalling pathway in endometrial cancer cells. The Ishikawa and HEC-1-B cells were treated with 0, 10 or 20 µM tetrandrine for 24 h. (A) The mRNA expression levels of Akt were measured by qRT-PCR. (B) The protein expression levels of p-Akt and Akt were detected by Western blotting. (C) The ratio of p-Akt/Akt was relative to control group (0 µM tetrandrine). #P<0.05. (D) Immunofluorescence staining of p-Akt was observed by a fluorescence microscope (scale bars: 50 μm). (E) Quantitative analysis of p-Akt immunofluorescence. Data were presented as means ± SD (n=3), #P<0.05.