Component	50 ml NPB	50 ml NPBt	300 ml NPBt	Stock component
0.1% Triton X-100	0 μl	500 μl	3,000 μl	10% Triton X-100 (*add first)
0.5 mM spermidine	125 μl	125 μl	750 μl	0.2 M spermidine
0.2 mM spermine	50 μl	50 μl	300 μl	0.2 M spermine
1x cOmplete protease inhibitors (CPI)	2,000 μl	0 μl	0 μl	25x CPI (one tablet in 2 ml water)
For 8 samples:	Prep 2 x 50 ml	Prep 6 x 50 ml or 1 x 300 ml