Figure 2.

Dose-dependent induction of apoptosis in T24 cells by oridonin. (a, b) Annexin V-FITC- and PI-stained cells were quantified by flow cytometry after the T24 cells were incubated with oridonin (0, 1, 2, or 3 μM). (c–e) p53 and cleaved caspase-3 protein levels in T24 cells were assessed after the cells were incubated with oridonin (0, 1, 2, or 3 μM). (f) BAX mRNA levels in T24 cells incubated with oridonin (0, 1, 2, or 3 μM) were determined. ^∗∗∗P < 0.001 and ^∗∗∗∗P < 0.0001 vs. the untreated control (0 μM).