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. 2021 Jul 5;2021:4340950. doi: 10.1155/2021/4340950

Figure 3.

Figure 3

Oridonin inactivates the AKT and ERK signaling pathways by inhibiting TRPM7 expression. (a–d) Western blotting was performed to determine the expression of TRPM7, p-AKT, and p-ERK after the T24 cells were treated with 0, 2, and 3 μM oridonin. (b–e) RT-PCR was performed to determine the mRNA expression of FOXO3 after T24 cells were treated with 0, 2, and 3 μM oridonin. (f) T24 cells were infected with TRPM7-overexpressing plasmids, and then, TRPM7, p-AKT, and p-ERK expression was examined after treatment with and without 3 μM oridonin. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 vs. the untreated control (0 μM).