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Desmin, ZO-1, N-cadherin, and Cx43 expression in human hearts. (a) Lysates from donor hearts that were normal (N) or failing (F) were isolated and used for western blots with anti-desmin, anti-Cx43, anti-N-cadherin, or anti-ZO-1. GAPDH antibody was the loading control. (b) qPCR was used to test for mRNA for desmin, Cx43, N-cadherin, and ZO-1. ∗p < 0.05 vs nonfailing heart.
