Figure 5.

NF-κB signaling pathway plays a role in mediating the LPS-induced inhibitory effect on SVCT2 functional expression. SH-SY5Y cells were pretreated with celastrol (100 nM) for 5 h before LPS treatment; then 48 h later, the NF-κB expression in the nucleus (a), IKKαβ expression in the cytoplasm (b), carrier-mediated AA uptake (c), hSVCT2 protein expression (d), hSVCT2 mRNA expression (e), and SLC23A2 promoter activity (f) were determined. @: cells exposed to LPS plus celastrol treatment significantly recovered versus LPS alone-treated cells. Values are means ± SE of at least 3 separate investigations. ^∗∗∗P < 0.001; ^∗∗P < 0.01; ^∗P < 0.05.