Table 1.
Overexpression of human SNCA leads to oxidative stress.
| Genotype | CAT | SOD | GSH | LPO | ROS |
|---|---|---|---|---|---|
| UAS-SNCAWT/+ | 6.7 ± 0.32 | 0.58 ± 0.01 | 19.2 ± 2.03 | 6.1 ± 0.24 | 689 ± 25.6 |
| elav/+; SNCAWT/+ | 4.5 ± 0.24 | 0.49 ± 0.02 | 11.5 ± 1.50 | 7.0 ± 0.85 | 844 ± 11.3 |
| p value | p < 0.01 | p < 0.01 | p < 0.01 | p < 0.01 | p < 0.01 |
| UAS-SNCAE46K/+ | 6.5 ± 0.27 | 0.62 ± 0.04 | 18.5 ± 1.34 | 5.0 ± 0.47 | 700 ± 33.1 |
| elav/+; SNCAE46K/+ | 4.4 ± 0.53 | 0.47 ± 0.03 | 12.8 ± 1.02 | 6.8 ± 0.26 | 895 ± 21.6 |
| p value | p < 0.01 | p < 0.01 | p < 0.01 | p < 0.01 | p < 0.01 |
Endogenous antioxidant enzyme activity and levels of oxidative markers are noticeably distorted and in SNCA transgenic PD model flies. Data represent mean ± SE of each biochemical marker. An independent sample t-test was applied, and corresponding p values indicate significant differences between tested genotypes. A total of 9 samples were analyzed in each genotype (n = 9). CAT = catalase-specific activity (μM H2O2/min/mg protein); SOD = superoxide-dismutase-specific activity (units/mg protein); GSH = glutathione levels (μg GSH/mg protein); LPO = lipid peroxidation levels (nM MDA/mg protein); ROS = reactive oxygen species levels (μM DCF/mg protein).