Curculigoside treatment promoted the osteogenic-associated gene expression in Ti-induced in MC3T3-E1 cells. (a) qRT-PCR analysis was performed to detect the relative mRNA expression of Runx2, osterix, and OCN in MC3T3-E1 cells. (b) Western blot analysis was performed to detect the relative protein expression of casepase-3, casepase-9, SIRT1, BMP-2, RANKL, and OPG in MC3T3-E1 cells (n = 3); results are represented as the mean ± SD. ▲P < 0.05, compared to the Con group; ▲▲P < 0.01, compared to the Con group; ∗P < 0.05, compared to the Ti group; ∗∗P < 0.01, compared to the Ti group. Ti: titanium particle; Con: control.