AM2201 and JWH-018 enhance 5-HT1A-mediated outward currents in DRN neurons obtained from CNR1−/− mice. a and b, Average time course (mean ± SEM) of 5-HT-induced change in holding current (pA) during perfusion with 3 μM 5-HT following 30 min pre-incubation with 10 μM AM2201, 10 μM JWH-018, or 0.1% DMSO vehicle. c, Peak change in holding current (pA) for individual cells for a and b. The mean and 95% confidence intervals are shown. (F(2,34) = 6.903, p = 0.003, one-way ANOVA; *p <0.05, **p<0.01, Dunnett’s post-hoc test vs. 0.1% DMSO).