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. 2021 Jun 28;11:669088. doi: 10.3389/fcimb.2021.669088

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Copyright © 2021 Cubillos, Prata, Fotoran, Ranford-Cartwright and Wunderlich

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PfAP2-O-GFP-HA-DD24 is expressed in schizont stage parasites. (A) Fluorescence microscopy of late schizont parasites shows that GFP-tagged PfAP2-O colocalizes with the DAPI (nuclear stain) signal. WGA marks the surface of red blood cells. (B) Western blot of cytoplasmic and nuclear fractions obtained from PfAP2-O-GFP-HA-DD24 expressing parasites shows that AP2-O-GFP-HA-DD24 is found exclusively in the nucleus of schizonts. The membrane containing the transferred proteins was cut in three parts and was probed with monoclonal anti-HA (upper part), polyclonal anti-TPK (middle part), and monoclonal anti-Histone 3 antibodies (lower part). See Supplementary Figures for the full-size exposure.