Figure 3.

var gene transcription analysis during a transient knockdown in PfAP2-O-GFP-HA-DD24 parasites. (A) RNAs extracted from NF54::PfAP2-O-GFP-DD24 parasites grown in the presence of Shield-1, and panned three times over CHO-CD36 cells were analyzed by RT-qPCR using t-seryl ligase (“K1”) as an internal control transcript, as described in Methods. (B) var transcript profile after 96 h incubation of parasites without Shield-1. (C) var transcript profile in parasites after re-establishment of PfAP2-O for two reinvasions (96 h). (D) var transcription profile in parasites never submitted to silencing of PfAP2-O (Always on Shield-1, at the end of the knockdown experiment). The data represent results from three independent experiments and error bars indicate the standard deviation between these.