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. 2021 Jun 28;11:669088. doi: 10.3389/fcimb.2021.669088

Figure 5.

Figure 5

PfAP2-O-GFP-HA-DD24 is involved in the transcription/translation of gametocyte-related genes in P. falciparum. (A) Transcript abundance of gametocyte-stage specific genes in PfAP2-O-GFP-HA-DD24 expressing, knocked-down (two reinvasions without Shield-1), and recovered parasites (two reinvasion cycles without Shield-1, followed by two reinvasion cycles in the presence of Shield-1). The data were normalized to seryl-tRNA ligase (PF3D7_0717700), used as the internal control. For statistical evaluation, the ANOVA test with Bonferroni’s correction was used. ** is p < 0.01 and *** is p < 0.005. (B) Western blot detecting Pfs16/Pf3D7_0406200 in extracts of NF54::PfAP2-O_GFP_HA_DD24 parasites in the three conditions (on Shield-1/knocked-down/recovered parasites). As a control, an anti-ATC antibody was used as before. See Supplementary Figures for the full size exposure.