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. 2021 Jun 28;11:669088. doi: 10.3389/fcimb.2021.669088

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Copyright © 2021 Cubillos, Prata, Fotoran, Ranford-Cartwright and Wunderlich

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PfAP2-O is essential for P. falciparum transmission to Anopheles coluzzii. Mosquitoes were fed with gametocytes of NF54 and NF54::AP2-O-GFP-DD24, induced in the absence of Shield-1 (see Methods and Supplementary Figure 3 for details). (A) Prevalence of infected mosquitoes fed with ApiAP2-O knockdown and WT gametocytes in each experimental feeding. (B) Predicted effect of parasite clone on mosquito infection, bars indicate 95% confidence intervals. (C) number of oocysts observed in each experimental feed. (D) Predicted effect of each clone in oocyst number. Bars indicate 95% confidence intervals.