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. 2021 Jul 12;12:4262. doi: 10.1038/s41467-021-24545-3

Fig. 5. Inhibitors targeting class I HDAC and BRD proteins reverse EMT.

Fig. 5

a Immunoblotting assay of EMT markers in treated T11 cells in 2D culture. Fimepinostat was applied at 200 nM and all other drugs were applied at 1 μM concentration. Representative images of independent biological triplicates. b Immunoblotting assay of EMT markers in treated T12 cells in 2D culture. Fimepinostat was applied at 20 nM and all other drugs were applied at 1 μM concentration. Representative images of independent biological triplicates. KDM histone lysine demethylase, BRD bromodomain, HDAC histone deacetylase. c qPCR of EMT marker genes in treated T11 cells. Drug concentrations as described in (a). Data are presented relative to Actb and shown as mean ± s.e.m. of independent biological triplicates. d qPCR of EMT marker genes in treated T12 cells. Drug concentrations as described in (b). Data are presented relative to Actb and shown as mean ± s.e.m. of independent biological triplicates. ns, not significant. Statistical analysis by unpaired Student t-tests (two-tailed).