Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 12;11:390. doi: 10.1038/s41398-021-01517-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Male C57BL/6 mice received a single IP injection of LPS or saline and microglia were isolated from whole brains at 7 days later. The nanoString Mouse Neuroinflammation Panel was used to determine differential mRNA expression. (A) Hierarchical clustering of the differentially expressed genes was assessed and significantly enriched biological pathways were identified by Ncounter system. A heatmap depicting the gene expression by category is depicted for the saline and LPS treatment at 7 days after administration. Orange indicates high expression; blue indicates low expression. (B) A volcano plots depicting the differential mRNA expression in 7 days LPS vs Saline. Values shown are the log2 (fold change) ratios of LPS treatment with respect to the saline group plotted against the −log10 (p-value). The black dots denote the analyzed genes with p-value below the given False Discovery Rate (FDR) or p-value threshold. The horizontal line indicates the False Discovery Rate (FDR) threshold or adjusted p-value <0.05 determined by the Benjamini–Yekutieli and the orange dots indicate the most statistically significant genes (p < 0.05). (C) The Venn diagram illustrates the overlap of significantly modified genes by treatment (n = 3).