Fig. 8. FEC + oHSV-1 + CP increases TIL-Bs and reduces immunosuppressive cell populations in E0771 tumors C57/Bl6 mice bearing E0771 tumors were treated with either an anti-CD20 mAB or isotype mAB, followed by treatment with saline, FEC + oHSV-1, or FEC + oHSV-1 + CP.

Mice were sacrificed on day 10, tumors were processed, and TILs were stained for analysis via flow cytometry. a Bar plots showing the frequency of B cells (CD19⁺) and memory B cells (CD19⁺CD27⁺) in the tumor. b Representative flow plot showing the gating strategy for MDSCs (Ly6G^hiLy6C^int) in mice treated with the isotype mAB (left) and the anti-CD20 mAB (right). c Bar plots showing the frequency of MDSCs in the tumor. d Bar plots showing the frequency of macrophages (F4/80⁺), CD244.2⁺ cells, and PDL1⁺ cells. e Representative flow plot showing the gating strategy for monocytes (Ly6C^hi) and MDSCs in splenocytes. f Bar plots showing the frequency of B cells (CD19⁺B220⁺), monocytes, and MDSCs in splenocytes. g Representative flow plot showing the gating strategy for MDSCs in TDLNs. h Bar plots showing the frequency of monocytes and MDSCs in TDLNs. i Bar plots showing the frequency of B cells and memory B cells in TDLNs. *Two-tail unpaired t test was used for statistical analyses. Error bars are representative of standard deviation.