Fig. 2. KI198^Cre expression is restricted to mature excitatory neurons in the hippocampus.

a Anti-EGFP (green) and anti-NeuN (red) immunofluorescent confocal images of coronal hippocampal sections at the level of dorsal (top left) and ventral (bottom left) hippocampus from a KI198^Cre::Rosa^fsTRAP mouse. Dorsal and ventral CA1 and CA3 fields (white boxes) are shown at higher magnification on the right. Scale bars: 500 µm (left panels) or 100 µm (right panels). b Anti-EGFP (green) and anti-NeuN (red) immunofluorescent confocal images of coronal sections at the level of dorsal (top left) and ventral (bottom left) hippocampus from an Htr4-bacTRAP mouse. Dorsal and ventral CA1 and CA3 fields (white boxes) are shown at higher magnification on the right. Scale bars: 500 µm (left panels) or 100 µm (right panels). c Immunofluorescent confocal images showing neuronal-type specific marker (red) and EGFP (green) expression in coronal sections through the DG of KI198^Cre::Rosa^fsTRAP mice. Cells labeled with a neuron-type marker but not EGFP (arrowheads) and cells double-labeled with hilar mossy cell marker CRT and EGFP (asterisks) are indicated. ml: molecular layer, gcl: granule cell layer. Scale bars, 50 µm. d Immunofluorescent confocal images of coronal sections through the DG of Htr4-bacTRAP mice labeled with anti-NeuN (red) and anti-EGFP (green). ml: molecular layer, gcl: granule cell layer. Scale bars, 50 µm.