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. 2021 Jul 12;11:14319. doi: 10.1038/s41598-021-93590-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The RNA transcriptome in heterogeneous between the brain and the spinal cord in different disease states. (A) Principal component analysis of RNA-seq expression profiles for microglia in the brain and the spinal cord and different disease stages. Separation was observed in the brain and the spinal at different disease stages. The variance in X-axis (PC1) is 76.5% and on Y axis (PC2) is 12.9%. (B) UpSeTR diagram showing the number of differentially expressed genes due to each combination of experimental factors using brain in naïve condition as baseline. Three animals per group were used in these experiments⁷¹.