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. 2021 Jul 12;12:4245. doi: 10.1038/s41467-021-24499-6

Fig. 6. Activation of RAGC is sufficient to re-localize TFEB into the cytoplasm in TSC2-deficient cells.

Fig. 6

a Immunofluorescent analysis of HeLa-TFEB-GFP cells after siRNA downregulation for 72 h transfected with wild-type (WT) RAGA plus WT RAGC vs. constitutively active (CA) RAGA (RAGA Q66L) plus CA RAGC (RAGC S75N) for 48 h (n = 3 biological replicates per condition). b Immunofluorescent analysis of HeLa-TFEB-GFP cells after TSC2 siRNA downregulation as in (a), and individually transfected with WT RAGA, CA RAGA, WT RAGC, or CA RAGC for 48 h (n = 3 biological replicates per condition). c Representative immunoblot analysis of cells treated as in (b) with indicated antibodies (n = 3 biological replicates per condition). d Working model in which TSC2 regulates TFEB cytoplasmic/nuclear localization via RAGC (created with BioRender.com). Scale bars = 50 µm. Source data are provided as a Source data file.