Fig. 1.

Recombinant PfHsp70-1 expression and purification analysis. PfHsp70-1 was over-expressed in E. coli BL21 (DE3) cells and successfully purified using nickel-affinity chromatography. Lane M, prestained dual colour protein marker; lane CL, control depicts untransformed cells; lane 0, pre-IPTG induction; lane 3 and 24 represent, 3 and 24 h post-IPTG induction samples. Lane W represent the unbound flow through, whereas lane E represents the elution fraction containing the purified protein. Western blot analysis was used to confirm the presence expression and purification of PfHsp70-1 using monoclonal anti-polyHistidine−peroxidase conjugated antibody (1:2000) produced in mouse on PVDF membrane block with 1 mg/ml BSA (lower panel)