Fig. 5.

Knock-down of TRPV4 with siRNA-attenuated NMDA-induced cytotoxicity and calcium accumulation in primary cultured hippocampal neurons. Neurons were pretreated with TRPV4 siRNA (10 nmol/L) for 24 h, and then exposed to NMDA (100 μmol/L) for 24 h. A Cell viability evaluated by MTT assays (mean ± SD, n = 6; *P <0.05 vs NC siRNA group, ^#P <0.05 vs NMDA 0 μmol/L group. B, C Intracellular Ca²⁺ concentrations measured using Fluo-4 Direct Calcium Assay kits (green, Fluo-4; blue, Hoechst; original magnification ×200; scale bars, 50 μm; mean ± SD, n = 7; *P <0.05 vs negative siRNA group, ^#P <0.05 vs NMDA group, ANOVA and Dunnett’s multiple comparison test).