FIGURE 5.

Combined treatment of BA and U0126 activates the AKT pathway and autophagy. (A) A549 and H1650 cells were treated with BA alone, U0126 alone, or in combination for 48 h, and the levels of AKT, p-AKT, LC3, cleaved PARP, ERK, and p-ERK1/2 were determined using Western blots. (B) A549 and H1650 cells were treated with BA alone, U0126 alone, HCQ alone, or the indicated combinations for 48 h, and apoptosis was measured by flow cytometry. (C) A549 and H1650 cells were treated with the indicated chemicals alone or in combination for 48 h, and the levels of AKT and p-AKT were evaluated by Western blots. (D) A549 and H1650 cells were treated with the indicated chemicals alone or combination of the chemicals for 48 h, and apoptosis was detected using flow cytometry. **p < 0.01.