Table 1.
Antioxidant enzymes relative gene expression in female and male aortas and brain blood vessels (BBV). Results are presented as relative expression of gene normalized to hypoxanthine-guanine phosphoribosyltransferase as a reference gene and summarized as arithmetic mean ± standard deviation (two-way ANOVA)
| Aortas |
Cu/Zn Sod* |
MnSod† |
EC-Sod‡ |
Gpx1§ |
Gpx4‖ |
Cat⁋ |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| female |
male |
female |
male |
female |
male |
female |
male |
female |
male |
female |
male |
|
| Control |
0.99 ± 0.64 (N = 4) |
0.57 ± 0.62 (N = 5) |
0.21 ± 0.41 (N = 5) |
0.10 ± 0.09 (N = 5) |
0.23 ± 0.29 (N = 5) |
0.72 ± 0.80 (N = 4) |
0.09 ± 0.05 (N = 5) |
0.70 ± 0.52*** (N = 5) |
0.19 ± 0.22 (N = 5) |
0.61 ± 0.43***
(N = 6) |
1.01 ± 0.82 (N = 5) |
1.45 ± 0.51 (N = 5) |
| Carbohydrate- and fat-rich diet (HSHFD) |
0.61 ± 0.34 (N = 6) |
0.34 ± 0.24 (N = 6) |
0.09 ± 0.08 (N = 7) |
0.08 ± 0.05 (N = 5) |
0.15 ± 0.08 (N = 6) |
0.37 ± 0.27 (N = 5) |
0.08 ± 0.04 (N = 7) |
0.2 ± 0.25 (N = 5) |
0.16 ± 0.12 (N = 7) |
0.56 ± 0.36 (N = 6) |
0.84 ± 0.51 (N = 5) |
0.56 ± 0.37 (N = 4) |
|
BBV |
Cu/Zn Sod** |
MnSod†† |
EC-Sod‡‡ |
Gpx1§§ |
Gpx4‖‖ |
Cat⁋⁋ |
||||||
| female |
male |
female |
male |
female |
male |
female |
male |
female |
male |
female |
male |
|
| Control |
0.39 ± 0.13 (N = 6) |
0.80 ± 0.06††† (N = 5) |
0.55 ± 0.20 (N = 6) |
0.46 ± 0.60 (N = 5) |
0.59 ± 0.34 (N = 6) |
1.13 ± 0.95 (N = 5) |
1.77 ± 0.71 (N = 6) |
0.73 ± 0.12††† (N = 5) |
0.69 ± 0.47 (N = 6) |
0.75 ± 0.28 (N = 5) |
0.85 ± 0.27 (N = 6) |
0.92 ± 0.11 (N = 5) |
| Carbohydrate- and fat-rich diet (HSHFD) | 0.21 ± 0.15 (N = 6) | 0.39 ± 0.13 (N = 5) | 0.02 ± 0.003 (N = 4) | 0.30 ± 0.16‡‡‡ (N = 6) | 0.046 ± 0.04 (N = 8) | 0.14 ± 0.09 (N = 5) | 0.09 ± 0.09 (N = 6) | 0.39 ± 0.30 (N = 6) | 0.06 ± 0.03 (N = 8) | 0.17 ± 0.05 (N = 5) | 0.82 ± 0.37 (N = 4) | 0.48 ± 0.47 (N = 5) |
*Cu/Zn Sod (P = 0.7194, F = 0.1337).
†MnSod (P = 0.5165, F = 0.4379).
‡EC-Sod (P = 0.4627, F = 0.5662).
§Gpx1 (P = 0.0285, F = 5.673).
‖‖Gpx4 (P = 0.5229, F = 0.4230)
¶Cat (P = 0.4006, F = 0.7485).
**Cu/Zn Sod (P = 0.0311, F = 5.471).
††MnSod (P = 0.0151, F = 7.300).
‡‡EC-Sod (P = 0.2579, F = 1.356).
§§Gpx1 (P = 0.0008, F = 15.98).
‖‖Gpx4 (P = 0.7992, F = 0.06643).
¶¶Cat (P = 0.1835, F = 1.933).
***Male aortas control vs female aortas control (Gpx1 P < 0.01; Gpx4 P < 0.05), Bonferroni post hoc test, P < 0.05.
†††Male BBV control vs female BBV control (Cu/Zn Sod P < 0.001; Gpx1 P < 0.05), Bonferroni post hoc test, P < 0.05.
‡‡‡Male BBV HSHFD vs female BBV HSHFD (MnSod P < 0.05), Bonferroni post hoc test, P < 0.05.